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Scientists Have First 3-D View of Life’s Processes in Liquid

October 14, 2019/in Science/by The National Digest

A new liquid-cell technology allows scientists to see living biological materials and systems in three dimensions under an electron microscope, according to researchers at Penn State, Virginia Tech and Protochips Inc.

“With this technology that we developed in collaboration with Protochips, scientists could analyze host-pathogen interactions, see a virus being introduced into a cell and watch molecular mechanisms take place in real time,” says Deb Kelly, professor of biomedical engineering. “The work represents the world’s first nanoscale CAT scan in a liquid environment.”

In a cover article appearing in Nano Letters, Kelly and colleagues report new insights into bacteriophage and host bacterium interactions that could in the future lead to methods to kill antibiotic-resistant bacteria. Their images revealed structural features of the bacteriophage that were previously not well understood.

The field of liquid-cell electron microscopy has grown rapidly in recent years, but until now it has been limited to 2-D. In tomography, slices of a sample are imaged as the sample is tilted. Then, the images are stacked into 3-D using computer software.

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